Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets

Doaa Assar; Zizy Elbialy; Rasha Al Wakeel; Naglaa Gomaa; Mahmoud Metwaly El-Maghraby; Wael Mohamed Nagy; Adel Abd El Aziz El-badawy; Abdel-Khalek El-Sayed Abdel-Khalek

doi:doi:10.11648/j.aap.20230801.12

Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets

Doaa Assar, Zizy Elbialy, Rasha Al Wakeel, Naglaa Gomaa, Mahmoud Metwaly El-Maghraby, Wael Mohamed Nagy, Adel Abd El Aziz El-badawy, Abdel-Khalek El-Sayed Abdel-Khalek

Published in Advances in Applied Physiology (Volume 8, Issue 1)

Received: 4 February 2023 Accepted: 22 February 2023 Published: 3 March 2023

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Recently, chitosan gained a great attention due to its unique biological activities as a natural biodegradable polymer derived from chitin with non-antigenic, non-toxic. It has several positive impacts on animal health including potent antioxidant, antimicrobial activities, and anti-immunogenicity. Therefore, it is a natural, bioactive, mucoadhesive, and biocompatible compound used commonly as a safe additive in animal production. This study was conducted to detect the effects of dietary inclusion of chitosan in high-fat diet (HFD) on growth, hematology, lipid profile, rumen function, oxidative stress, and antioxidant status of Zaraibi goat bucks. Total of 18 sexually mature bucks (38.69±0.57 kg BW) were allocated into 3 groups (n= 6); the control group fed the control diet and treatment groups received HFD (the control diet with 3% fat) and the HFD plus 2.5% chitosan for 8 weeks, respectively. Results showed that HFD increase (P<0.05) final body weight, total weight gain, white blood cells (WBCs), and serum total cholesterol (TC), triglycerides (TG), VLDL, LDL, and malondialdehyde (MDA) with declined free T4 hormone, and HDL with the exhaustion of GSH, CAT and GPx activities beside reducing ruminal total proteins, glucose, ammonia-N, TVFA, total and L-lactate concentrations. Chitosan dietary inclusion to HFD reversed the aforementioned parameters with a notable enhancement of the antioxidant enzyme activities, suppressed the elevated MDA levels, and restored the depleted T4 level. Therefore, chitosan could be safely utilized as a dietary supplement in buck's diets to improve organ functions, lipid profile, antioxidant defense system, scavenge free radicals, and potentiate Buck's reproductive activities.

Published in	Advances in Applied Physiology (Volume 8, Issue 1)
DOI	10.11648/j.aap.20230801.12
Page(s)	8-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Chitosan, High Fat Diet, Lipid Metabolism, Antioxidant, Leukocytes, Zaraibi Goat Bucks

References

[1]	Bunnoy, A., Saenphet, K., Lumyong, S., Saenphet, S., and Chomdej, S. (2015). Monascus purpureus-fermented Thai glutinous rice reduces blood and hepatic cholesterol and hepatic steatosis concentrations in diet-induced hypercholesterolemic rats. BMC Complement. Altern. Med. 15: 88.
[2]	Ouchi, N., Parker, J. L., Lugus, J. J., and Walsh, K. (2011). Adipokines in inflammation and metabolic disease. Nat. Rev. Immunol., 11: 85–97.
[3]	Grummer, R. R. (1993). Etiology of lipid-related metabolic disorders in periparturient dairy cows. J. Dairy Sci. 76: 3882–3896. https://doi.org/10.3168/jds.S0022-0302(93)77729-2.
[4]	Chiu, C. Y., Yen, T. E., Liu, S. H., and Chiang, M. T. (2019). Comparative effects and mechanisms of chitosan and its derivatives on hypercholesterolemia in high-fat diet-fed rats. International journal of molecular sciences, 21 (1), 92. https://doi.org/10.3390/ijms21010092
[5]	Dawood, M. A. O., Ali, M. F., Amer, A. A., Gewaily, M. S., Mahmoud, M. M., Alkafafy, M., Assar, D. H., Soliman, A. A., and Doan, H. V. (2021). The influence of coconut oil on the growth, immune and anti-oxidative responses and the intestinal digestive enzymes and histomorphometry features of Nile tilapia (Oreochromis niloticus) Fish Physiol Biochem https://doi.org/10.1007/s10695-021-00943-8
[6]	Tu, H., Yu, Y., Chen, J., Shi, X., Zhou, J., Deng, H., and Du, Y. (2017). Highly cost-effective and high-strength hydrogels as dye adsorbents from natural polymers: Chitosan and cellulose. Polym. Chem. 8, 2913–2921.
[7]	Okawa, H., Wijayagunawardane, M. M. P., Vos PLAM, Yamato, O., Taniguchi, M., and Takagi, M. (2021). Effects of intrauterine infusion of a chitosan solution on recovery and subsequent reproductive performance of early postpartum dairy cows with endometritis: a pilot field trial. Animals, 11, 197. doi: https://doi.org/10.3390/ ani11010197.
[8]	Li, Z., Lei, X., Chen, X., Yin, Q., Shen, J., and Yao, J. (2021). Long-term and combined effects of N-[2-(nitrooxy) ethyl]-3-pyridinecarboxamide and fumaric acid on methane production, rumen fermentation, and lactation performance in dairy goats. J. Anim. Sci. Biotechnol. 12, 1–12.
[9]	Shah, A. M., Qazi, I. H., Matra, M., and Wanapat, M. (2022). Role of Chitin and Chitosan in Ruminant Diets and Their Impact on Digestibility, Microbiota and Performance of Ruminants. Fermentation. 8, 549. https://doi.org/10.3390/fermentation8100549
[10]	Boguslawski, S., Bunzeit, M., and Knorr, D. (1990). Effects of chitosan treatment on clarity and microbial counts of apple juice, Zeitschrift für Lebensmittel-Technologie undVerfahrenstechnik 41. 42–44.
[11]	Seankamsorn, A., Cherdthong, A., and Wanapat, M. (2020). Combining crude glycerin with chitosan can manipulate in vitro ruminal efficiency and inhibit methane synthesis. Animals, 10, 37. https://doi.org/10. 3390/ani10010037.
[12]	Abd El-Hack, M. E., El-Saadony, M. T., Shafi, M. E., Zabermawi, N. M., Arif, M., Batiha, G. E., Khafaga, A. F., Abd El-Hakim, Y. M., and Al-Sagheer, A. A. (2020). Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: A review. Int. J. Biol. Macromol., 164: 2726–2744.
[13]	Chiu, C. Y. Feng, S. A., Liu, S. H. and. Chiang, M. T. (2017). Functional Comparison for Lipid Metabolism and Intestinal and Fecal Microflora Enzyme Activities between Low Molecular Weight Chitosan and Chitosan Oligosaccharide in High-Fat-Diet-Fed Rats, Mar. Drugs, 15, 234.
[14]	Bagheri-Khoulenjani, S., Taghizadeh, S. M., and Mirzadeh, H. (2009). An investigation on the short-term biodegradability of chitosan with various molecular weights and degrees of deacetylation, Carbohydr. Polym. 78 (4) 773–778.
[15]	NRC, (2007). Nutrient Requirements of Small Ruminants: Sheep, Goats, Cervids, and New World Camelids. Washington DC: National Academy Press.
[16]	Bernard, L., Toral, P. G., and Chilliard, Y. (2017). Comparison of mammary lipid metabolism in dairy cows and goats fed diets supplemented with starch, plant oil, or fish oil. Journal of dairy science, 100 (11), 9338–9351. https://doi.org/10.3168/jds.-12789
[17]	Chiu, C. Y., Chan, I. L, Yang, T. H., Liu, S. H., and Chiang, M. T. (2015). Supplementation of chitosan alleviates high-fat diet-enhanced lipogenesis in rats via adenosine monophosphate (AMP)-activated protein kinase activation and inhibition of lLipogenesis-associated genes. Journal of Agricultural and Food Chemistry, 63 (11), 2979–2988. doi: 10.1021/acs.jafc.5b00198.
[18]	Feldman, B. F., Zinkl, J. G., and Jain, N. C. (2000). Schalm’s veterinary hematology. 5th ed. Philadelphia, USA: Lippincott Williams & Wilkins. 21-100.
[19]	Richmond, W. (1973). Enzymatic determination of cholesterol. Clin. Chem. 19: 1350.
[20]	Abell, l. L., Levy, B. B., Brodie, B. B., and Kendall, F. E. (1952). A simplified method for the estimation of total cholesterol in serum. J. B1iol. Chem., 195: 357-366.
[21]	Friedewald, W. T., Levy, R. I., and Fredriekson, D. S. (1972). Estimation of concentration of low density lipoprotein cholesterol in plasma without use of preparative ultracentrifuge. Clin Chem. 18: 499–502.
[22]	Ohkawa, H., Ohishi, N., and Yagi, K. (1979). Assay tor lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochem, 95: 351-358.
[23]	Beutler, E., Duron, O., and Kelly, B. M. (1963). Improved method for the determination of blood glutathione. J Lab Clin.
[24]	Aebi, H. (1984). Catalase in vitro, Methods in Enzymology, Elsevier, pp. 121–126.
[25]	Paglia, D. E., and Valentine, W. N. (1967). Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Med. 70 (1): 158-69.
[26]	Faix, J. D., and Miller, W. G. (2016). Progress in standardizing and harmonizing thyroid function tests. Am J Clin Nutr. 104 Suppl 3: 913s-917s.
[27]	Van-Soest, J. P. (1991). The use of detergents in the analysis of fibrous feeds. Determination of plant constituents. Journal of Association of Agriculture Chemistry. 50: 50-55.
[28]	Lorenz, I., Hartmann, I., and Gentile, A. (2003). Determination of d-lactate in calf serum samples: an automatedenzymatic assay. Comp. Clin. Pathol., 12: 169–171.
[29]	Liu, J., Zhang, J., and Xia, W. (2008). Hypocholesterolaemic effects of different chitosan samples in vitro and in vivo. Food Chem. 107, 419–425.
[30]	Sun, Z. H., Tang, Z. R., Yin, Y. L., Huang, R. L., Li, T. J., and Tang, S. X. (2009). Effect of dietary supplementation of galacto-mannan-oligosaccharides and chitosan on performance and serum immune parameters of 28-day weaned piglets challenged with pathogenic E. coli. J. Appl. Anim. Res. 36, 207–211.
[31]	Liu, S. H., Chen, R. Y., and Chiang, M. T. (2021). Effects and Mechanisms of Chitosan and ChitosanOligosaccharide on Hepatic Lipogenesis and Lipid Peroxidation, Adipose Lipolysis, and Intestinal Lipid Absorption in Rats with High-Fat Diet-Induced Obesity. International journal of molecular sciences, 22 (3), 1139. https://doi.org/10.3390/ijms22031139
[32]	Xu, Y. Q., Shi, B. L., Yan, S. M., Li, T. Y., Guo, Y. W., and Li, J. L. (2013). Effects of chitosan on body weight gain, growth hormone and intestinal morphology in weaned pigs. Asian Austral. J. Anim., 26, 1484–1489. doi: 10.5713/ajas. 13085.
[33]	Xu, Y., Shi, B., Yan, S., Li, J., Li, T., and Guo, Y., (2014). Effects of chitosan supplementation on the growth performance, nutrient digestibility, and digestive enzyme activity in weaned pigs. Czech J. Anim. Sci. 59, 156–163.
[34]	Guan, G., Azad, MAK., Lin, Y., Kim, S. W., Tian, Y., Liu, G. and Wang, H. (2019). Biological Effects and Applications of Chitosan and Chito-Oligosaccharides. Front. Physiol. 10: 516. doi: 10.3389/fphys.00516.
[35]	Del Valle, T. A., Paiva, P. G., Jesus, E. F., Almeida, G. F., Costa, A. G. B. V. B., Bueno, I. C. S., and Rennó, F. P. (2017). Dietary chitosan improves nitrogen use and feed conversion in diets for midlactation dairy cows. Livestock Science, 201, 22-29.
[36]	Andersen, C. J., Murphy, K. E., and Fernandez, M. L. (2016). Impact of Obesity and Metabolic Syndrome on Immunity. Adv. Nutr., 7: 66–75.
[37]	Aigner, E., Feldman, A., and Datz, C. (2014). Obesity as an emerging risk factor for iron deficiency. Nutrients.; 6 (9): 3587–600.
[38]	Cepeda-lopez, A. C., Aeberli, I., and Zimmermann, M. B. (2010). Does Obesity Increase Risk for Iron Deficiency? A Review of the Literature and the Potential Mechanisms. Int. J. Vitam. Nutr. Res.; 80 (4–5): 263–70.
[39]	Schütz, F., Figueiredo-Braga, M., Barata, P., and Cruz-Martins, N. (2021). Obesity and gut microbiome: review of potential role of probiotics. Porto biomedical journal, 6 (1), e111. https://doi.org/10.1097/j.pbj.0000000000000111
[40]	Lee, J. Y., Kim, T. Y., Kang, H., Oh, J., Park, J. W., Kim, S. C., Kim, M., Apostolidis, E., Kim, Y. C., and Kwon, Y. I. (2021). Anti-Obesity and Anti-Adipogenic Effects of Chitosan Oligosaccharide (GO2KA1) in SD Rats and in 3T3-L1 Preadipocytes Models. Molecules (Basel, Switzerland), 26 (2), 331. https://doi.org/10.3390/molecules26020331
[41]	Stern, J. H., Rutkowski, J. M., and Scherer, P. E. (2016). Adiponectin, leptin, and fatty acids in the maintenance of metabolic homeostasis through adipose tissue crosstalk. Cell Metab. 23, 770–784.
[42]	Eckel, R. H., Grundy, S. M., and Zimmet, P. Z. (2005). The metabolic syndrome. Lancet. 365: 1415-28; PMID: 15836891; http:// dx.doi.org/10.1016/S0140-6736(05)66378-7
[43]	Kerch, G. (2015). The potential of chitosan and its derivatives in prevention and treatment of age-related diseases. Mar. Drugs 13, 2158–2182.
[44]	Sumiyoshi, M., Sakanaka, M., and Kimura, Y. (2006). Chronic intake of high-fat and high-sucrose diets differentially affects glucose intolerance in mice. The Journal of nutrition, 136 (3), 582–587. https://doi.org/10.1093/jn/136.3.582
[45]	Zong, C., Yu, Y., Song, G., Luo, T., Li, L., Wang, X., and Qin, S. (2012). Chitosan oligosaccharides promote reverse cholesterol transport and expression of scavenger receptor BI and CYP7A1 in mice. Exp. Biol. Med. 237, 194–200.
[46]	Pan, H., Yang, Q., Huang, G., Ding, C., Cao, P., Huang, L., Xiao, T., Guo, J., and Su, Z. (2016). Hypolipidemic effects of chitosan and its derivatives in hyperlipidemic rats induced by a high-fat diet. Food Nutr. Res. 60, 31137.
[47]	Yao, H. T., Huang, S. Y., and Chiang, M. T. (2008). A comparative study on hypoglycemic and hypocholesterolemic effects of high and low molecular weight chitosan in streptozotocin-induced diabetic rats. Food Chem. Toxicol. 46, 1525–1534.
[48]	Choi, E. H., Yang, H. P., and Chun, H. S. (2012). Chitooligosaccharide ameliorates diet-induced obesity in mice and affects adipose gene expression involved in adipogenesis and inflammation. Nutr. Res. 2012, 32, 218–228.
[49]	Yao, H. T., Luo, M. N. and Li, C. C. (2015). Chitosan oligosaccharides reduce acetaminophen-induced hepatotoxicity by suppressing CYP-mediated bioactivation. J. Funct. Foods. 12, 262–270.
[50]	Kakino, S., Ohki, T., Nakayama, H., Yuan, X., Otabe, S., Hashinaga, T., Wada, N., Kurita, Y., Tanaka, K., and Hara, K. (2018). Pivotal Role of TNF-α in the Development and Progression of Nonalcoholic Fatty Liver Disease in a Murine Model. Horm. Metab. Res. 50, 80–87.
[51]	Yin, Y. L., Tang, Z. R., Sun, Z. H., Liu, Z. Q., Li, T. J., and Huang, R. L. (2008). Effect of galacto-mannan-oligosaccharides or chitosan supplementation on cytoimmunity and humoral immunity in early-weaned piglets. Asian Austral. J. Anim. 21, 723–731.
[52]	Wan, J., Jiang, F., Xu, Q. S., Chen, D. W., Yu, B., and Huang, Z. Q., (2017). New insights into the role of chitosan oligosaccharide in enhancing growth performance, antioxidant capacity, immunity and intestinal development of weaned pigs. RSC Adv. 7, 9669–9679.
[53]	Noeman, S. A., Hamooda, H. E., and Baalash, A. A. (2011). Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetol. Metab. Syndr. 3, 17.
[54]	Mohammadi A. A, et al. (2015). Effects of probiotics on biomarkers of oxidative stress and inflammatory factors in petrochemical workers: a randomized, double-blind, placebo- controlled trial. Int. J. Prev. Med. 6: 82. doi: 10.4103/2008-7802.164146.
[55]	Lasker, S., Rahman, M. M., Parvez, F., Zamila, M., Miah, P., Nahar, K., Kabir, F., Sharmin, S. B., Subhan, N., Ahsan, G. U., and Alam, M. A. (2019). High-fat diet-induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation. Scientific Reports, 9 (1), 20026. https://doi.org/10.1038/s41598-019-56538-0
[56]	Wang, B., Zhang, S., Wang, X., Yang, S., Jiang, Q., Xu, Y., and Xia, W. (2017). Transcriptome analysis of the effects of chitosan on the hyperlipidemia and oxidative stress in high-fat diet fed mice. Int. J. Biol. Macromol. 102: 104–110.
[57]	Anraku, M., Fujii, T., Furutani, N., Kadowaki, D., Maruyama, T., Otagiri, M., Gebicki, M. J., and Tomida, H. (2009). Antioxidant effects of a dietary supplement: reduction of indices of oxidative stress in normal subjects by water-soluble chitosan, Food Chem. Toxicol. 47 (1) 104–109.
[58]	Gudla, P., AbuGhazaleh, A. A., Ishlak, A., and Jones, K., (2012). The effect of level of forage 641 and oil supplement on biohydrogenation intermediates and bacteria in continuous 642 cultures. Anim. Feed Sci. Technol. 171, 108-116.
[59]	Shingfield, K. J., Lee, M. R. F., Humphries, D. J., Scollan, N. D., Toivonen, V., Reynolds, 753 C. K., and Beever, D. E., (2010). Effect of incremental amounts of fish oil in the diet on 754 ruminal lipid metabolism in growing steers. Brit. J. Nutr. 104, 56-66.
[60]	Jenkins, T. C. (1993). Lipid Metabolism in the Rumen, 1993 J Dairy Sci. 76: 3851-3863.
[61]	Yang, S., Bu, D., Wang, J., Hu, Z., Li, D., Wei, H., Zhou, L., and Loor, J. (2009). Soybean oil and linseed oil supplementation affect profiles of ruminal microorganisms in dairy cows. Anim. 3, 1562–1569.
[62]	Goiri, I., Oregui, L. M., and Garcia-Rodriguez, A. (2010). Use of chitosans to modulate ruminal fermentation of 50: 50 forage-to-concentrate diet in sheep. J. Anim. Sci., 88, 749-755.
[63]	Araújo, A. P. C. d., Venturelli, B. C., Santos, M. C. B., Gardinal, R., Cônsolo, N. R. B., Calomeni, G. D., Freitas, J., Barletta, R. V., Gandra, J. R., and Paiva, P. (2015). Chitosan affects total nutrient digestion and ruminal fermentation in Nellore steers. Anim. Feed. Sci. Technol. 206, 114–118.
[64]	Cherdthong, A. (2020). Potential use of rumen digesta as ruminant diet—A review. Tropical Animal Health and Production, 52, 1-6.
[65]	Chen, H. C., Chang, C. C., Mau, W. J., and Yen, L. S. (2002). Evaluation of N-acetylchitooligosaccharides as the main carbon sources for the growth of intestinal bacteria. FEMS Microbiol. Lett., 209, 53–56.
[66]	Clarke, S. F., Murphy, E. F., Nilaweera, K., Ross, P. R., Shanahan, F., O’Toole, P. W., and Cotter, P. D. (2012). The gut microbiota and its relationship to diet and obesity: new insights. Gut Microbes. 3: 186-202; PMID: 22572830; http://dx.doi.org/ 10.4161/gmic.20168
[67]	Jonsson, A. L., and Backhed, F. (2017). Role of gut microbiota in atherosclerosis. Nat Rev Cardiol; 14: 79-87.
[68]	Zhang, X., Chen, W., Shao, S., Xu, G., Song, Y., Xu, C., Gao, L., Hu, C., and Zhao, J. (2018). A High-Fat Diet Rich in Saturated and Mono-Unsaturated Fatty Acids Induces Disturbance of Thyroid Lipid Profile and Hypothyroxinemia in Male Rats. Molecular Nutrition & Food Research, 62, & NA.
[69]	Kanaya, A. M., Harris, F., Volpato, S., Pérez-Stable, E. J., Harris, T., and Bauer, D. C. (2002). Association between thyroid dysfunction and total cholesterol level in an older biracial population: the health, aging and body composition study. Arch Intern Med. 162: 773.
[70]	Lee, M. H., Lee, J. U. Joung, K. H., Kim, Y. K., Ryu, M. J., Lee, S. E., Kim, S. J., Chung, H. K., Choi, M. J., Chang, J. Y., Lee, S.-H., Kweon, G. R., Kim, H. J., Kim, K. S., Kim, S.-M., Jo, Y. S., Park, J., Cheng, S.-Y., and Shong, M. (2015). Endocrinology, 156, 1181.
[71]	Ebrahim, R. M. (2020). Prophylactic effect of Spirulina platensis on radiation-induced thyroid disorders and alteration of reproductive hormones in female albino rats. International Int. J. Radiat. Res., 18 (1): 83-90. doi: 10.18869/acadpub.ijrr.18.1.83.

Cite This Article

Plain Text BibTeX RIS

APA Style

Doaa Assar, Zizy Elbialy, Rasha Al Wakeel, Naglaa Gomaa, Mahmoud Metwaly El-Maghraby, et al. (2023). Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets. Advances in Applied Physiology, 8(1), 8-16. https://doi.org/10.11648/j.aap.20230801.12

Copy | Download

ACS Style

Doaa Assar; Zizy Elbialy; Rasha Al Wakeel; Naglaa Gomaa; Mahmoud Metwaly El-Maghraby, et al. Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets. Adv. Appl. Physiol. 2023, 8(1), 8-16. doi: 10.11648/j.aap.20230801.12

Copy | Download

AMA Style

Doaa Assar, Zizy Elbialy, Rasha Al Wakeel, Naglaa Gomaa, Mahmoud Metwaly El-Maghraby, et al. Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets. Adv Appl Physiol. 2023;8(1):8-16. doi: 10.11648/j.aap.20230801.12

Copy | Download

@article{10.11648/j.aap.20230801.12,
  author = {Doaa Assar and Zizy Elbialy and Rasha Al Wakeel and Naglaa Gomaa and Mahmoud Metwaly El-Maghraby and Wael Mohamed Nagy and Adel Abd El Aziz El-badawy and Abdel-Khalek El-Sayed Abdel-Khalek},
  title = {Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets},
  journal = {Advances in Applied Physiology},
  volume = {8},
  number = {1},
  pages = {8-16},
  doi = {10.11648/j.aap.20230801.12},
  url = {https://doi.org/10.11648/j.aap.20230801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20230801.12},
  abstract = {Recently, chitosan gained a great attention due to its unique biological activities as a natural biodegradable polymer derived from chitin with non-antigenic, non-toxic. It has several positive impacts on animal health including potent antioxidant, antimicrobial activities, and anti-immunogenicity. Therefore, it is a natural, bioactive, mucoadhesive, and biocompatible compound used commonly as a safe additive in animal production. This study was conducted to detect the effects of dietary inclusion of chitosan in high-fat diet (HFD) on growth, hematology, lipid profile, rumen function, oxidative stress, and antioxidant status of Zaraibi goat bucks. Total of 18 sexually mature bucks (38.69±0.57 kg BW) were allocated into 3 groups (n= 6); the control group fed the control diet and treatment groups received HFD (the control diet with 3% fat) and the HFD plus 2.5% chitosan for 8 weeks, respectively. Results showed that HFD increase (P<0.05) final body weight, total weight gain, white blood cells (WBCs), and serum total cholesterol (TC), triglycerides (TG), VLDL, LDL, and malondialdehyde (MDA) with declined free T4 hormone, and HDL with the exhaustion of GSH, CAT and GPx activities beside reducing ruminal total proteins, glucose, ammonia-N, TVFA, total and L-lactate concentrations. Chitosan dietary inclusion to HFD reversed the aforementioned parameters with a notable enhancement of the antioxidant enzyme activities, suppressed the elevated MDA levels, and restored the depleted T4 level. Therefore, chitosan could be safely utilized as a dietary supplement in buck's diets to improve organ functions, lipid profile, antioxidant defense system, scavenge free radicals, and potentiate Buck's reproductive activities.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets
AU  - Doaa Assar
AU  - Zizy Elbialy
AU  - Rasha Al Wakeel
AU  - Naglaa Gomaa
AU  - Mahmoud Metwaly El-Maghraby
AU  - Wael Mohamed Nagy
AU  - Adel Abd El Aziz El-badawy
AU  - Abdel-Khalek El-Sayed Abdel-Khalek
Y1  - 2023/03/03
PY  - 2023
N1  - https://doi.org/10.11648/j.aap.20230801.12
DO  - 10.11648/j.aap.20230801.12
T2  - Advances in Applied Physiology
JF  - Advances in Applied Physiology
JO  - Advances in Applied Physiology
SP  - 8
EP  - 16
PB  - Science Publishing Group
SN  - 2471-9714
UR  - https://doi.org/10.11648/j.aap.20230801.12
AB  - Recently, chitosan gained a great attention due to its unique biological activities as a natural biodegradable polymer derived from chitin with non-antigenic, non-toxic. It has several positive impacts on animal health including potent antioxidant, antimicrobial activities, and anti-immunogenicity. Therefore, it is a natural, bioactive, mucoadhesive, and biocompatible compound used commonly as a safe additive in animal production. This study was conducted to detect the effects of dietary inclusion of chitosan in high-fat diet (HFD) on growth, hematology, lipid profile, rumen function, oxidative stress, and antioxidant status of Zaraibi goat bucks. Total of 18 sexually mature bucks (38.69±0.57 kg BW) were allocated into 3 groups (n= 6); the control group fed the control diet and treatment groups received HFD (the control diet with 3% fat) and the HFD plus 2.5% chitosan for 8 weeks, respectively. Results showed that HFD increase (P<0.05) final body weight, total weight gain, white blood cells (WBCs), and serum total cholesterol (TC), triglycerides (TG), VLDL, LDL, and malondialdehyde (MDA) with declined free T4 hormone, and HDL with the exhaustion of GSH, CAT and GPx activities beside reducing ruminal total proteins, glucose, ammonia-N, TVFA, total and L-lactate concentrations. Chitosan dietary inclusion to HFD reversed the aforementioned parameters with a notable enhancement of the antioxidant enzyme activities, suppressed the elevated MDA levels, and restored the depleted T4 level. Therefore, chitosan could be safely utilized as a dietary supplement in buck's diets to improve organ functions, lipid profile, antioxidant defense system, scavenge free radicals, and potentiate Buck's reproductive activities.
VL  - 8
IS  - 1
ER  -

Copy | Download

Author Information

Doaa Assar

Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
Zizy Elbialy

Department of Fish Processing and Biotechnology, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Kafrelsheikh, Egypt
Rasha Al Wakeel

Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt
Naglaa Gomaa

Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelshiekh University, Kafrelsheikh, Egypt
Mahmoud Metwaly El-Maghraby

Animal Production Research Institute, Agricultural Research Center, Giza, Egypt
Wael Mohamed Nagy

Animal Production Research Institute, Agricultural Research Center, Giza, Egypt
Adel Abd El Aziz El-badawy

Animal Production Research Institute, Agricultural Research Center, Giza, Egypt
Abdel-Khalek El-Sayed Abdel-Khalek

Animal Production Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Doaa Assar, Zizy Elbialy, Rasha Al Wakeel, Naglaa Gomaa, Mahmoud Metwaly El-Maghraby, et al. (2023). Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets. Advances in Applied Physiology, 8(1), 8-16. https://doi.org/10.11648/j.aap.20230801.12

Copy | Download

ACS Style

Doaa Assar; Zizy Elbialy; Rasha Al Wakeel; Naglaa Gomaa; Mahmoud Metwaly El-Maghraby, et al. Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets. Adv. Appl. Physiol. 2023, 8(1), 8-16. doi: 10.11648/j.aap.20230801.12

Copy | Download

AMA Style

Doaa Assar, Zizy Elbialy, Rasha Al Wakeel, Naglaa Gomaa, Mahmoud Metwaly El-Maghraby, et al. Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets. Adv Appl Physiol. 2023;8(1):8-16. doi: 10.11648/j.aap.20230801.12

Copy | Download

@article{10.11648/j.aap.20230801.12,
  author = {Doaa Assar and Zizy Elbialy and Rasha Al Wakeel and Naglaa Gomaa and Mahmoud Metwaly El-Maghraby and Wael Mohamed Nagy and Adel Abd El Aziz El-badawy and Abdel-Khalek El-Sayed Abdel-Khalek},
  title = {Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets},
  journal = {Advances in Applied Physiology},
  volume = {8},
  number = {1},
  pages = {8-16},
  doi = {10.11648/j.aap.20230801.12},
  url = {https://doi.org/10.11648/j.aap.20230801.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20230801.12},
  abstract = {Recently, chitosan gained a great attention due to its unique biological activities as a natural biodegradable polymer derived from chitin with non-antigenic, non-toxic. It has several positive impacts on animal health including potent antioxidant, antimicrobial activities, and anti-immunogenicity. Therefore, it is a natural, bioactive, mucoadhesive, and biocompatible compound used commonly as a safe additive in animal production. This study was conducted to detect the effects of dietary inclusion of chitosan in high-fat diet (HFD) on growth, hematology, lipid profile, rumen function, oxidative stress, and antioxidant status of Zaraibi goat bucks. Total of 18 sexually mature bucks (38.69±0.57 kg BW) were allocated into 3 groups (n= 6); the control group fed the control diet and treatment groups received HFD (the control diet with 3% fat) and the HFD plus 2.5% chitosan for 8 weeks, respectively. Results showed that HFD increase (P<0.05) final body weight, total weight gain, white blood cells (WBCs), and serum total cholesterol (TC), triglycerides (TG), VLDL, LDL, and malondialdehyde (MDA) with declined free T4 hormone, and HDL with the exhaustion of GSH, CAT and GPx activities beside reducing ruminal total proteins, glucose, ammonia-N, TVFA, total and L-lactate concentrations. Chitosan dietary inclusion to HFD reversed the aforementioned parameters with a notable enhancement of the antioxidant enzyme activities, suppressed the elevated MDA levels, and restored the depleted T4 level. Therefore, chitosan could be safely utilized as a dietary supplement in buck's diets to improve organ functions, lipid profile, antioxidant defense system, scavenge free radicals, and potentiate Buck's reproductive activities.},
 year = {2023}
}

Copy | Download

TY  - JOUR
T1  - Dietary Chitosan Supplementation Modulates Hematology, Lipid Profile, Rumen Function, Antioxidant Status, and Thyroxin in Zaraibi Goat Bucks Fed on High-Fat Diets
AU  - Doaa Assar
AU  - Zizy Elbialy
AU  - Rasha Al Wakeel
AU  - Naglaa Gomaa
AU  - Mahmoud Metwaly El-Maghraby
AU  - Wael Mohamed Nagy
AU  - Adel Abd El Aziz El-badawy
AU  - Abdel-Khalek El-Sayed Abdel-Khalek
Y1  - 2023/03/03
PY  - 2023
N1  - https://doi.org/10.11648/j.aap.20230801.12
DO  - 10.11648/j.aap.20230801.12
T2  - Advances in Applied Physiology
JF  - Advances in Applied Physiology
JO  - Advances in Applied Physiology
SP  - 8
EP  - 16
PB  - Science Publishing Group
SN  - 2471-9714
UR  - https://doi.org/10.11648/j.aap.20230801.12
AB  - Recently, chitosan gained a great attention due to its unique biological activities as a natural biodegradable polymer derived from chitin with non-antigenic, non-toxic. It has several positive impacts on animal health including potent antioxidant, antimicrobial activities, and anti-immunogenicity. Therefore, it is a natural, bioactive, mucoadhesive, and biocompatible compound used commonly as a safe additive in animal production. This study was conducted to detect the effects of dietary inclusion of chitosan in high-fat diet (HFD) on growth, hematology, lipid profile, rumen function, oxidative stress, and antioxidant status of Zaraibi goat bucks. Total of 18 sexually mature bucks (38.69±0.57 kg BW) were allocated into 3 groups (n= 6); the control group fed the control diet and treatment groups received HFD (the control diet with 3% fat) and the HFD plus 2.5% chitosan for 8 weeks, respectively. Results showed that HFD increase (P<0.05) final body weight, total weight gain, white blood cells (WBCs), and serum total cholesterol (TC), triglycerides (TG), VLDL, LDL, and malondialdehyde (MDA) with declined free T4 hormone, and HDL with the exhaustion of GSH, CAT and GPx activities beside reducing ruminal total proteins, glucose, ammonia-N, TVFA, total and L-lactate concentrations. Chitosan dietary inclusion to HFD reversed the aforementioned parameters with a notable enhancement of the antioxidant enzyme activities, suppressed the elevated MDA levels, and restored the depleted T4 level. Therefore, chitosan could be safely utilized as a dietary supplement in buck's diets to improve organ functions, lipid profile, antioxidant defense system, scavenge free radicals, and potentiate Buck's reproductive activities.
VL  - 8
IS  - 1
ER  -

Copy | Download