Fasting blood glucose is used to measure metabolic status and is important in the diagnosis and management of diabetes. Its study has continued to generate attention due to the increase in diabetes globally. Most of the reported studies have been on humans. This study, therefore, investigated the effects of sex and weights on fasting glucose levels in mice and rats. Forty-eight male and female albino rats weighing between 50-200g and twenty mice weighing between 16-28g were used for the study. The animals were acclimatized for two weeks. They were fasted overnight, and blood sample was collected from each animal for estimation of blood glucose. Their weights were measured, and blood glucose was determined by modified glucose oxidase method. The results of this study showed that male mice and rats have significantly heavier body weights and higher levels of fasting blood glucose compared with female rats and mice. The study shows that sex and weight affect fasting blood glucose levels in mice and rats. In conclusion, the finding of this study suggests that sex and weight should be considered for accurate assessment of metabolic status in mice and rats. This observation may also be relevant to the assessment of glucose metabolism in humans.
| Published in | Advances in Applied Physiology (Volume 8, Issue 1) |
| DOI | 10.11648/j.aap.20230801.15 |
| Page(s) | 29-31 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2024. Published by Science Publishing Group |
Fasting Blood Glucose, Sex, Body Weight, Rats, Mice
| [1] | O. Varlamov, C. L. Bethea, and C. T. Roberts Jr, “Sex-specific differences in lipid and glucose metabolism,” Front. Endocrinol., vol. 5, p. 241. |
| [2] | F. Mauvais-Jarvis, “Sex differences in metabolic homeostasis, diabetes, and obesity,” Biol. Sex Differ., vol. 6: 14, 2015. |
| [3] | F. Mauvais-Jarvis, “Gender differences in glucose homeostasis and diabetes,” Physiol. Behav., vol. 187, pp. 20–23, 2018. |
| [4] | M. S. Hedrington and S. N. Davis, “Sexual dimorphism in glucose and lipid metabolism during fasting, hypoglycemia, and exercise,” Front. Endocrinol., vol. 6, p. 61, 2015. |
| [5] | V. Lagou et al,“Sex-dimorphic genetic effects and novel loci for fasting glucose and insulin variability,” Nat. Commun., vol. 12, no. 1, p. 24, 2021. |
| [6] | L. Perreault et al., “Sex differences in diabetes risk and the effect of intensive lifestyle modification in the Diabetes Prevention Program,” Diabetes Care, vol. 31, no. 7, pp. 1416–1421, 2008. |
| [7] | K. Faerch, K. Borch-Johnsen, A. Vaag, T. Jørgensen, and D. R. Witte, “Sex differences in glucose levels: a consequence of physiology or methodological convenience? The Inter99 study,” Diabetologia, vol. 53, pp. 858–865, 2010. |
| [8] | R. A. Sicree, P. Z. Zimmet, D. W. Dunstan, A. J. Cameron, T. A. Welborn, and J. E. Shaw, “Differences in height explain gender differences in the response to the oral glucose tolerance test—the AusDiab study,” Diabet. Med., vol. 25, no. 3, pp. 296–302, 2008. |
| [9] | H. Yan et al., “Estrogen improves insulin sensitivity and suppresses gluconeogenesis via the transcription factor Foxo1,” Diabetes, vol. 68, no. 2, pp. 291–304, 2019. |
| [10] | B. Tramunt et al., “Sex differences in metabolic regulation and diabetes susceptibility,” Diabetologia, vol. 63, pp. 453–461, 2020. |
| [11] | A. Kautzky-Willer, J. Harreiter, and G. Pacini, “Sex and gender differences in risk, pathophysiology and complications of type 2 diabetes mellitus,” Endocr. Rev., vol. 37, no. 3, pp. 278–316, 2016. |
| [12] | C. Gustavsson et al., “Sex-different hepaticglycogen content and glucose output in rats,” BMC Biochemistry vol. 11, no. 38., 2010. |
| [13] | J. D. Wagner, K. Kavanagh, G. M. Ward, B. J. Auerbach, H. J. Harwood Jr, and J. R. Kaplan, “Old world nonhuman primate models of type 2 diabetes mellitus,” ILAR J., vol. 47, no. 3, pp. 259–271, 2006. |
| [14] | F. Yue, G. Zhang, R. Tang, Z. Zhang, L. Teng, and Z. Zhang, “Age-and sex-related changes in fasting plasma glucose and lipoprotein in cynomolgus monkeys,” Lipids Health Dis., vol. 15, no; 111, pp. 1–10, 2016. |
| [15] | D. Tripathy et al., “Insulin secretion and insulin sensitivity in relation to glucose tolerance: lessons from the Botnia Study.,” Diabetes, vol. 49, no. 6, pp. 975–980, 2000. |
| [16] | J. W. Williams et al., “Gender differences in the prevalence of impaired fasting glycaemia and impaired glucose tolerance in Mauritius. Does sex matter?,” Diabet. Med., vol. 20, no. 11, pp. 915–920, 2003. |
| [17] | C. J. Chiu, L. A. Wray, and E. A. Beverly, “Relationship of glucose regulation to changes in weight: a systematic review and guide to future research,” Diabetes Metab. Res. Rev., vol. 26, no. 5, pp. 323–335, 2010. |
| [18] | M. S. Islam, “Fasting blood glucose and diagnosis of type 2 diabetes,” Diabetes Res. Clin. Pract., vol. 91, no. 1, p. e26, 2011. |
| [19] | D. L. Palliyaguru et al., “Fasting blood glucose as a predictor of mortality: Lost in translation,” Cell Metab., vol. 33, no. 11, pp. 2189-2200. e3, 2021. |
| [20] | E. Trinder, “Determination of blood glucose using 4 amino phenazone as oxygen acceptor,” J. Chem. Pathol, vol. 22; 246”. 1969. |
| [21] | P. Vital, E. Larrieta, and M. Hiriart, “Sexual dimorphism in insulin sensitivity and susceptibility to develop diabetes in rats,” J. Endocrinol., vol. 190, no. 2, pp. 425–432, 2006. |
| [22] | A. Nadal et al., “Rapid insulinotropic effect of 17β-estradiol via a plasma membrane receptor,” FASEB J., vol. 12, no. 13, pp. 1341–1348, 1998. |
| [23] | A. B. Ropero, B. Soria, and A. Nadal, “A nonclassical estrogen membrane receptor triggers rapid differential actions in the endocrine pancreas,” Mol. Endocrinol., vol. 16, no. 3, pp. 497–505, 2002. |
| [24] | X. Wu et al., “Relationships between body weight, fasting blood glucose concentration, sex and age in tree shrews (Tupaia belangeri chinensis),” J. Anim. Physiol. Anim. Nutr., vol. 97, no. 6, pp. 1179–1188, 2013. |
| [25] | R. Basu et al., “Effects of pioglitazone and metformin on NEFA-induced insulin resistance in type 2 diabetes,” Diabetologia, vol. 51, pp. 2031–2040, 2008. |
| [26] | H. Karakelides, B. A. Irving, K. R. Short, P. O’Brien, and K. S. Nair, “Age, obesity, and sex effects on insulin sensitivity and skeletal muscle mitochondrial function,” Diabetes, vol. 59, no. 1, pp. 89–97, 2010. |
| [27] | P. Nuutila et al., “Gender and insulin sensitivity in the heart and in skeletal muscles: studies using positron emission tomography,” Diabetes, vol. 44, no. 1, pp. 31–36, 1995. |
| [28] | S. H. Khan, U. Masood, M. S. Hanif, S. O. R. S. Bokhari, and M. J. Khan, “Effect of age and gender on blood lipids and glucose,” Rawal Med. J., vol. 37, no. 4, pp. 344–344, 1970. |
APA Style
Isehunwa Grace Olufunmilayo, Shittu Sheu-Tijani, Apeji Comfort Oiza, Ayedun Mojisola Temidayo. (2023). Effects of Sex and Weight on Fasting Blood Glucose in Mice Mus musculus, and Rats Rattus norvegicus. Advances in Applied Physiology, 8(1), 29-31. https://doi.org/10.11648/j.aap.20230801.15
ACS Style
Isehunwa Grace Olufunmilayo; Shittu Sheu-Tijani; Apeji Comfort Oiza; Ayedun Mojisola Temidayo. Effects of Sex and Weight on Fasting Blood Glucose in Mice Mus musculus, and Rats Rattus norvegicus. Adv. Appl. Physiol. 2023, 8(1), 29-31. doi: 10.11648/j.aap.20230801.15
AMA Style
Isehunwa Grace Olufunmilayo, Shittu Sheu-Tijani, Apeji Comfort Oiza, Ayedun Mojisola Temidayo. Effects of Sex and Weight on Fasting Blood Glucose in Mice Mus musculus, and Rats Rattus norvegicus. Adv Appl Physiol. 2023;8(1):29-31. doi: 10.11648/j.aap.20230801.15
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Download@article{10.11648/j.aap.20230801.15,
author = {Isehunwa Grace Olufunmilayo and Shittu Sheu-Tijani and Apeji Comfort Oiza and Ayedun Mojisola Temidayo},
title = {Effects of Sex and Weight on Fasting Blood Glucose in Mice Mus musculus, and Rats Rattus norvegicus},
journal = {Advances in Applied Physiology},
volume = {8},
number = {1},
pages = {29-31},
doi = {10.11648/j.aap.20230801.15},
url = {https://doi.org/10.11648/j.aap.20230801.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aap.20230801.15},
abstract = {Fasting blood glucose is used to measure metabolic status and is important in the diagnosis and management of diabetes. Its study has continued to generate attention due to the increase in diabetes globally. Most of the reported studies have been on humans. This study, therefore, investigated the effects of sex and weights on fasting glucose levels in mice and rats. Forty-eight male and female albino rats weighing between 50-200g and twenty mice weighing between 16-28g were used for the study. The animals were acclimatized for two weeks. They were fasted overnight, and blood sample was collected from each animal for estimation of blood glucose. Their weights were measured, and blood glucose was determined by modified glucose oxidase method. The results of this study showed that male mice and rats have significantly heavier body weights and higher levels of fasting blood glucose compared with female rats and mice. The study shows that sex and weight affect fasting blood glucose levels in mice and rats. In conclusion, the finding of this study suggests that sex and weight should be considered for accurate assessment of metabolic status in mice and rats. This observation may also be relevant to the assessment of glucose metabolism in humans.},
year = {2023}
}
TY - JOUR T1 - Effects of Sex and Weight on Fasting Blood Glucose in Mice Mus musculus, and Rats Rattus norvegicus AU - Isehunwa Grace Olufunmilayo AU - Shittu Sheu-Tijani AU - Apeji Comfort Oiza AU - Ayedun Mojisola Temidayo Y1 - 2023/06/10 PY - 2023 N1 - https://doi.org/10.11648/j.aap.20230801.15 DO - 10.11648/j.aap.20230801.15 T2 - Advances in Applied Physiology JF - Advances in Applied Physiology JO - Advances in Applied Physiology SP - 29 EP - 31 PB - Science Publishing Group SN - 2471-9714 UR - https://doi.org/10.11648/j.aap.20230801.15 AB - Fasting blood glucose is used to measure metabolic status and is important in the diagnosis and management of diabetes. Its study has continued to generate attention due to the increase in diabetes globally. Most of the reported studies have been on humans. This study, therefore, investigated the effects of sex and weights on fasting glucose levels in mice and rats. Forty-eight male and female albino rats weighing between 50-200g and twenty mice weighing between 16-28g were used for the study. The animals were acclimatized for two weeks. They were fasted overnight, and blood sample was collected from each animal for estimation of blood glucose. Their weights were measured, and blood glucose was determined by modified glucose oxidase method. The results of this study showed that male mice and rats have significantly heavier body weights and higher levels of fasting blood glucose compared with female rats and mice. The study shows that sex and weight affect fasting blood glucose levels in mice and rats. In conclusion, the finding of this study suggests that sex and weight should be considered for accurate assessment of metabolic status in mice and rats. This observation may also be relevant to the assessment of glucose metabolism in humans. VL - 8 IS - 1 ER -
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