Volume 3, Issue 1, June 2018, Page: 26-32
Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study
Pavol Svorc, Department of Physiology, Medical Faculty Safarik’s University, Kosice, Slovak Republic
Darina Petrasova, Laboratory of Research Bio-models, Medical Faculty Safarik’s University, Kosice, Slovak Republic
Pavol Svorc Jr., Department of Physiology and Patophysiology, Medical Faculty Ostrava University, Ostrava, Czech Republic
Received: May 27, 2018;       Accepted: Jun. 8, 2018;       Published: Jul. 9, 2018
DOI: 10.11648/j.aap.20180301.14      View  638      Downloads  33
Abstract
The design and development of experimental, in vivo, chronobiological animal models may help reveal some of the relationships between circadian rhythms and biological functions. In vivo experiments require the use of appropriate anaesthesia, which should be selected according to their particular effect on the organism. The aim of study was to review the status of acid-base balance and ion concentration in arterial blood under common used general anaesthesias in experiments in dependence on the light-dark (LD) cycle in spontaneously breathing rats. The experiments were performed using three- to four-month-old pentobarbital (P)-, ketamine/xylazine (K/X)- and zoletil (Z)-aneasthetized female Wistar rats after a four-week adaptation to an LD cycle (12h light:12h dark). It was concluded that P anaesthesia disturbs LD dependence of acid-base balance compared to K/X and Z anaesthesia, but LD differences in plasma ion concentrations are disturbed under all type of general anaesthesia. P anaesthesia is not the most appropriate type of anaesthesia in rat chronobiological experiments. It eliminated LD differences, and also produces a more acidic environment, more pronounced hypercapnia and hypoxia than K/X and Z anaesthesias. This should be taken into account because the altered internal environment may affect the activity of systems whose functions are primarily dependent on acid-base balance or/and ion concentrations.
Keywords
Chronobiology, Anaesthesia, Acid-Base Balance, Ions, Rats
To cite this article
Pavol Svorc, Darina Petrasova, Pavol Svorc Jr., Acid-Base Balance and Arterial Ion Concentrations in Rat Under Three Types of General Anaesthesia: Chronobiological Study, Advances in Applied Physiology. Vol. 3, No. 1, 2018, pp. 26-32. doi: 10.11648/j.aap.20180301.14
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Subramanian RK, Sidharthan A, Maneksh D, Ramalingam L, Soosai Manickam A, Kanthakumar P, Subramani S (2013) Normative data for arterial blood gas and electrolytes in anesthetized rats (Letter). Ind J Pharmacol 45: 103-104.
[2]
De Oliveira RB, De Macedo DV, Santos GB, Arcanjo AM (2012) Reliability of the electrocardiogram in normal rats. International Journal of Exercise Science: Conference Proceedings 1: Article 63.
[3]
Lewis LD, Ponten U, Siesjo BK (1973) Arterial acid-base changes in un-anesthetized rats in acute hypoxia. Respir Physiol 19: 312–21.
[4]
Pepelko WE, Dixon GA (1975) Arterial blood gases in conscious rats exposed to hypoxia, hypercapnia, or both. J Appl Physiol 38: 581-587.
[5]
Brun-Pascaud M, Gaudebout C, Blayo MC, Pocidalo JJ (1982) Arterial blood gases and acid-base status in awake rats. Respir Physiol 48: 45–57.
[6]
Girard P, Brun-Pascaud M, Pocidalo JJ (1983) Acid-base status of awake rats after cannulation of aorta and vena cava. Kidney Int 24: 795-799.
[7]
Hess L, Dvořáček I, Svobodník J (1988) Anesthesia of laboratory animals, Prague: Avicenum. Chapter 3, Rats (Rodentia); p. 158.
[8]
Dettmers Ch, Hagendorff A, Kastrup A, Luderitz B, Hartmann A (1994) An experimental model for hemodynamic evaluation of arrhythmias in rats. Cerebrovasc Dis 4: 309–313.
[9]
Chi OZ, Wei HM, Tse J, Klein SL, Weiss HR (1996) Cerebral microregional oxygen balance during chronic versus acute hypertension in middle cerebral artery occluded rats. Anesth Analg 82: 587–592.
[10]
Ohoi I, Takeo S (1996) Involvement of superoxide and nitric oxide in the genesis of reperfusion arrhythmias in rats. Eur J Pharmacol 306: 123–131.
[11]
Schultz JJ, Hsu AK, Gross GJ (1997) Ischemic preconditioning and morphine-induced cardioprotection involve the delta (δ)-opioid receptor in the intact rat heart. J Mol Cell Cardiol 29: 2187–2195.
[12]
Sun W, Wainwright CL (1997) The role of nitric oxide in modulating ischaemia-induced arrhythmias in rats. J Cardiovasc Pharmacol 29: 554–562.
[13]
Forkel J, Chen X, Wandinger S, Keser F, Duschin A, Schwanke U, Frede S, Massoudy P, Schulz R, Jakob H, Heusch G (2004) Responses of chronically hypoxic rat hearts to ischemia: KATP channel blockade does not abolish increased RV tolerance to ischemia. Am J Physiol Heart Circ Physiol 286: H545–H551.
[14]
Valenza F, Pizzocri M, Salice V, Chevallard G, Fossali T, Coppola S, Froio S, Polli F, Gatti S, Fortunato F, Comi GP, Gattinoni L (2012) Sodium bicarbonate treatment during transient or sustained lactic acidemia in normoxic and normotensive rats. PLoS ONE 7: e46035.
[15]
Peralta-Ramírez A, Raya AI, Pineda C, Rodríguez M, Aguilera-Tejero E, López I (2014) Acid-base balance in bremic bats with bascular balcification. Nephron Extra 4: 89–94.
[16]
Luo X, Yin Y, You G, Chen G, Wang Y, Zhao J, Wang B, Zhao L, Zhou H (2015) Gradually increased oxygen administration improved oxygenation and mitigated oxidative stress after resuscitation from severe hemorrhagic shock. Anesthesiology 123: 1122-1132.
[17]
Menegon LF, igueiredo JF, Gontijo JAR (1998) Effect of metabolic acidosis on renal tubular sodium handling in rats as determined by lithium clearance. Braz J Med Biol Res 31: 1269-1273.
[18]
Da Silva Costa EC, Gonçalves AA, Areas MA, Morgabel RGB (2008) Effects of metformin on QT and QTc interval dispersion of diabetic rats. Arq Bras Cardiol 90: 232-238.
[19]
Kim JA, Choi HJ, Kwon YK, Ryu DH, Kwon TH, Hwang GS (2014) 1H NMR-based metabolite profiling of plasma in a rat model of chronic kidney disease. PLoS ONE 9: e85445.
[20]
Dispersyn G, Pain L, Challet E, Touitou Y (2008) General anesthetics effects on circadian temporal structure: An Update. Chronobiol Int 25: 835-850.
[21]
Reinberg A (1986) Circadian rhythms in effects of hypnotics and sleep inducer. Int J Clin Pharmacol Res 6: 33–44.
[22]
Sato Y, Seo N, Kobahashi E (2005) The dosing-time dependent effects of intravenous hypnotics in mice. Anesth Analg 101: 1706–1708.
[23]
Haskins SC, Patz JD, Farver T. (1986) Xylazine and xylazine-ketamine in dogs. Am J Vet Res 47: 636–641.
[24]
Farver TB, Haskins SC, Patz JD (1986) Cardiopulmonary effects of acepromazine and of the subsequent administration of ketamine in the dog. Am J Vet Res 47: 631–635.
[25]
Kaczmarczyk G, Reinhardt HW (1975) Arterial blood gas tensions and acid-base status of wistar rats during thiopental and halothane anesthesia. Lab Anim Sci 25: 184–190.
[26]
Mortola JP, Seifert EL (2000) Hypoxic depression of circadian rhythms in adult rats. J Appl Physiol. 88: 365–368.
[27]
Bishop B, Silva G, Krasney J, Nakano H, Roberts A, Farkas G, Rifkin D, Shucard D (2001) Ambient temperature modulates hypoxic-induced changes in rat body temperature and activity differentially. Amer J Physiol 280: R1190–R1196.
[28]
Bosco G, Ionadi A, Panico S, Faralli F, Gagliardi R, Data P, Mortola JP (2003) Effects of hypoxia on the circadian patterns in men. High Alt Med Biol 4: 305–318.
[29]
Kaplan JL, Gao E, Garavilla L, Victain M, Minczak B, Dalsey WC (2003) Adenosine A1 antagonism attenuates atropine-resistant hypoxic bradycardia in rats. Acad Emerg Med 10: 923–930.
[30]
Mortola JP (2007) Hypoxia and circadian patterns. Respir Physiol Neurobiol 158: 274–279.
[31]
Prudian F, Gantenbein M, Pelissier AL, Attolini L, Bruguerolle B (1997) Daily rhythms of heart rate temperature and locomotor activity are modified by anaesthetics in rats: a telemetric study. NS Arch Pharmacol 355: 774–778.
[32]
Pelissier AL, Gantenbein M, Prudian F, Bruguerolle B (1988) Influence of general anaesthetics on circadian rhythms of heart rate, body temperature and locomotor activity in rats. Sci Tech Ani Lab 23: 91–98.
[33]
Fraley DS, Adler S (1976) Isohydric regulation of plasma potassium by bicarbonate in the rat. Kidney International 9: 333-343. https://doi.org/10.1038/ki.1976.39.
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